Scientists Boost Recombinant Protein Production in Soybean Seeds
May 12, 2021| |
Scientists from Jilin Academy of Agricultural Sciences and Jilin Normal University in China used polypeptide fusion strategy to study the capacity of the elastin-like polypeptide (ELP) and γ-zein fusions in boosting the production of recombinant protein in soybean seeds. The results are published in Transgenic Research.
Soybeans are considered as an optimal host for the production of recombinant proteins due to their high protein content, long-term stability of seed proteins under normal conditions, and easy establishment of efficient purification protocols. Thus, the researchers conducted the study and generated transgenic soybeans expressing γ-zein- or ELP-fused green fluorescent protein (GFP). They observed that there were significant differences in the accumulation of zein-GFP and GFP-ELP from that of the unfused GFP in transgenic soybean seeds based on the total soluble protein. However, there were no significant changes in total protein and oil content in seeds, implying that there could be threshold limits for such traits.
Read more findings in Transgenic Research.
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