PCM DNA Isolation Technique to Increase DNA Yield
October 8, 2014 |
Researchers from University of Wisconsin-Madison evaluated the efficacy of a new DNA isolation technique for plant tissues in terms of yield and purity of isolated DNA. The technique is based on MagnaCel paramagnetic cellulose particles (PMC), a method applied on forensic sciences which requires small amount of DNA. A comparative assessment of PCM with two other DNA isolation technique methods, silica column technique (DNeasy Plant Mini Kit) and cetyltrimethylammonium bromide (CTAB) method in terms of yield and purity of isolated DNA was conducted in 25 plant species.
The findings of the assessment show that PCM obtain twice higher DNA yield compared to CTAB and DNeasy and produces consistent DNA purity based on its absorbance ratios of 260:280 and 260:230 nm. These results provide evidence that PCM is more efficient and can be a useful tool in DNA isolation especially under low DNA concentrations.
Read more about this study at: http://www.bioone.org/doi/pdf/10.3732/apps.1400048.
|
Biotech Updates is a weekly newsletter of ISAAA, a not-for-profit organization. It is distributed for free to over 22,000 subscribers worldwide to inform them about the key developments in biosciences, especially in biotechnology. Your support will help us in our mission to feed the world with knowledge. You can help by donating as little as $10.
-
See more articles:
-
News from Around the World
- Governments Agree on Decisions Regarding Safe Use of LMOs
- Agricultural Innovations Can Help African Farmers Compete, Says Report
- Maui Mayor: Proposed Moratorium on GM Crops is Impractical
- Researchers Disable SDP1 Enzyme to Increase Oil Accumulation in Plant Leaves
- China Launches Media Campaign on Biotech Crops
- Scientists Reveal How Fall Armyworms Survive Plants' Defense
-
Research Highlights
- Scientists to Improve Disease Resistance of Sweet Oranges
- PCM DNA Isolation Technique to Increase DNA Yield
- Get to Know the BiP Genes from Bread Wheat
-
Beyond Crop Biotech
- International Team Reveals Monarch Butterfly's Genetic Secrets
- Recombinant Viral Vector Expressing hBD4 Inhibits Bacterial Infection in Burn Wounds
-
From the BICs
- Scientists and Policymakers Urge Students to Engage on Agri Research
- Thai BIC Organizes Two Biotech Communication Workshops
-
Announcements
- BIO Europe 2014
-
Resources
- Straight from the Scientists: Biotech Experts' Perspectives and Experiences in Informing the Public
-
Read the latest: - Biotech Updates (April 24, 2024)
- Gene Editing Supplement (April 24, 2024)
- Gene Drive Supplement (February 22, 2023)
-
Subscribe to BU: - Share
- Tweet