New LAMP System for Detection of GM Crops
November 13, 2013| |
Scientist Gurinder Jit Randhawa of the National Bureau of Plant Genetic Resources, India, and colleagues developed a new loop-mediated isothermal amplification (LAMP) system for screening GM crops. The optimized LAMP assays using designed primers, target commonly used promoters, such as Cauliflower Mosaic Virus 35S and Figwort Mosaic Virus promoter and marker genes aminoglycoside-3'-adenytransferase, neomycin phosphotransferase II and β-glucuronidase. The performance and specificity of the end-point and real-time LAMP assays were confirmed using 8 GE cotton events on four detection systems. LAMP assays on isothermal real-time system were found to be most sensitive, detecting up to four target copies, in just 35 minutes. Thus, the LAMP assays tested can be used for fast and cheep screening of GM status of a sample, regardless of the crop species of GM trait. These assays may be used with fast and simple DNA extraction technique for on-site GMO screening.
Read the research article at http://pubs.acs.org/doi/abs/10.1021/jf4030085.
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