Shoot Tip Transformation Technology for Maize
September 12, 2012| |
A research team from Tianjin University, China developed an effective and simple technology for maize genetic transformation. Using shoot meristems of two different maize inbred lines including maternal tian tawu and 7922. Phytoene synthase (psy) gene was transferred into maize by Agrobacterium-mediated transformation.
Factors influencing transformation efficiency were optimized in this research. The results indicated that the optimal transformation condition is infection time with vacuum at 20 min and co-cultivation time for 3 days. RT-PCR and HPLC analysis results showed that psy gene was integrated into the maize genome. The total carotenoid content in transgenic maize increased 25% compared to the wild type. This method eliminates the tedious process of tissue culture and proves to be a simple transformation method.
See the paper published by China Biotechnology at http://159.226.100.150:8082/biotech/CN/volumn/home.shtml. For more information visit http://www.chinabic.org.
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