Developer:
University of Saskatchewan

Method of Trait Introduction:
Agrobacterium tumefaciens-mediated plant transformation

GM Trait s :
Sulfonylurea herbicide tolerance , Antibiotic resistance , Nopaline synthesis

Commercial Trait:
(Singular)  Herbicide Tolerance

Summary of Basic Genetic Modification

Gene Introduced	Gene Source	Product	Function
als	Arabidopsis thaliana	herbicide tolerant enzyme acetolactate synthase (als)	allows the synthesis of essential amino acids in the presence of sulfonylurea herbicides
nos  *	Agrobacterium tumefaciens strain CP4	nopaline synthase enzyme	catalyses the synthesis of nopaline, which permits the identification of transformed plant embryos
nptII  *	Escherichia coli Tn5 transposon	neomycin phosphotransferase II enzyme	allows transformed plants to metabolize neomycin and kanamycin antibiotics during selection
bla  *	Escherichia coli	beta lactamase enzyme	detoxifies beta lactam antibiotics such as ampicillin
spc  *	Escherichia coli	spectinomycin adenyl transferase enzyme (not expressed in plant tissues)	confers resistance to spectinomycin/streptomycin antibiotics, which permits prokaryotic selection

 * : Selection Marker/Reporter  

Summary of Regulatory Approvals: Country, Year and Type of Approval

Country	Food direct use or processing	Feed direct use or processing	Cultivation domestic or non-domestic use
Canada
Colombia
United States of America

 

Event Description

• Biosafety Scanner
• CBD Biosafety Clearing House
• CERA GM Crop Database

Regulatory and Biosafety Information

• Canadian Food Inspection Agency
• US Department of Agriculture - APHIS
• US Food and Drug Administration
• US Food and Drug Administration

Detection Methods

• GMO Detection Method Database